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Active MEG3 upregulates p53 and p53 target genes. HRF was performed in triplicates for each condition, following a protocol described previously for group II intron (. Capture hybridization analysis of RNA targets (CHART). Long noncoding RNA MEG3 interacts with p53 protein and regulates partial p53 target genes in hepatoma cells. Protein repeats: structures, functions, and evolution. Imaging and force probing RNA by atomic force microscopy. ), You can customize by clicking on the images below. For cell cycle analysis, cells were incubated with EdU and the incorporation was detected using the Click-iT Plus EdU Flow Cytometry Assay Kit (Thermo Fisher Scientific) following manufacturer’s instructions with some modifications. Separately it was shown that the pressure gain between ear canal and scala vestibuli was a remarkably flat and smooth function of frequency. Sequence Alignments of v1, Related to Figure 2, Accepted: Définition, traduction, prononciation, anagramme et synonyme sur le dictionnaire libre Wiktionnaire. Atomic force microscopy of long and short double-stranded, single-stranded and triple-stranded nucleic acids. Approach to an irregular time-series on the basis of the fractal theory. Detection of RNA-protein interactions in living cells with SHAPE. Maternally expressed gene 3, an imprinted noncoding RNA gene, is associated with meningioma pathogenesis and progression. xlsx files, Download .zip (.02 A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. 1% agarose gels were run in 1x Tris-Borate (TB) buffer (89 mM Tris base, 89 mM boric acid) supplemented with the indicated concentrations of Mg, Analytical ultracentrifugation (AUC) sedimentation velocity experiments were performed as described (, Dynamic light scattering (DLS) was performed as previously described (. (E) qRT-PCR and western blot analysis of p53 upregulation by v1, v9, H11LpA, and G, (F) qRT-PCR analysis measuring upregulation of p53 target genes (BAX, p21, GADD45A, GDF15, and NOXA) by v1, v9, H11LpA, and G, (E and F) Error bars indicate SEM of 2 biological replicas, each performed in technical triplicates, and the black line indicates that the right and left parts of the images were manually joined because they were separated from each other in the raw image of the blot. Séparée de son fils, parmi ces femmes abîmées, surveillantes ou prisonnières, Ombline se construit en tant que femme, en tant que mère… Elle va se battre dans le seul espoir de récupérer la garde de Lucas à sa sortie de prison…. p53 gene mutations in pituitary adenomas: rare events. Identification of an imprinted gene, Meg3/Gtl2 and its human homologue MEG3, first mapped on mouse distal chromosome 12 and human chromosome 14q. Version Française disponible ici Prénom Emmii Donnez une note sur 5 à votre prénom en cliquant les étoiles ci-dessous : Les informations recueillies sont destinées à CCM Benchmark Group pour vous assurer l'envoi de votre newsletter. DOI: https://doi.org/10.1016/j.molcel.2019.07.025.
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